22nd Annual UltraScan Analytical Ultracentrifugation Workshop 2025

August 19-21, 2025, in Missoula, Montana, at the University of Montana Campus

Sponsored by:
Beckman Coulter, Thermo Fisher, AUC Solutions and the Northern Rockies Center for Hydrodynamics at the University of Montana
Solution Studies of Biological Macromolecules:
From Experimental Design to Data Analysis - UltraScan-III Workshop
Instructors:
Dr. Borries Demeler, Professor, Canada 150 Research Chair for Biophysics, University of Lethbridge, University of Montana
Sophia Bird, Dept. of Chemistry and Biochemistry, University of Lethbridge, Canadian Center for Hydrodynamics
Cindee Yates-Hansen, ISBC, University of Montana, Northern Rockies Center for Hydrodynamics
Morning Session (9:00 am - 12:30 pm)
ROOM Skaggs 174
Afternoon Session (1:30 pm - 5:00 pm)
ROOM Skaggs 174
Session 1: Tuesday, 8/19/25 - UltraScan Analysis Basics
  • Registration
  • UltraScan overview
  • UltraScan-in-a-Box account setup
  • ---- Coffee Break ----
  • UltraScan data import and the LIMS system
  • UltraScan integration with the Optima AUC
  • UltraScan data acquisition (GMP and R&D)
  • ---- Lunch ----
Session 2: Tuesday, 8/19/25 - UltraScan Analysis Basics
  • How to optimize your experimental design
  • Understanding noise in data
  • ---- Coffee Break ----
  • Editing experimental data
  • Fitting velocity data (2DSA, PCSA, van Holde - Weischet)
Session 3: Wednesday, 8/20/25 - AAV Analysis by Sedimentation Velocity
  • Analysis strategies by sedimentation velocity
  • Spectral Analysis
  • Multi-wavelength sedimentation velocity
  • GMP Reporting
  • ---- Coffee Break ----
  • GMP Data acquisition for single wavelength experiments
  • GMP Protocol development
  • ---- Lunch ----
  • Group Picture
Session 4: Wednesday, 8/20/25 - AAV - ABDE Experiments
  • ABDE (Analytical Buoyant Density Equilibrium) concepts
  • Experimental designs
  • ---- Coffee Break ----
  • GMP Data acquisition from disk and from Optima AUC
  • GMP AAV-ABDE workflow
Session 5: Thursday, 8/21/25 - Lipid Nanoparticles I
  • Custom grid analysis - concepts and applications
  • ---- Coffee Break ----
  • D2O Density matching experiments in UltraScan
  • Experimental design and analysis
  • Frictional ratio constraints
  • ---- Lunch ----
Session 6: Thursday, 8/21/25 - Lipid Nanoparticles II
  • Hands-on LNP analysis by custom grid with PCSA and 2DSA
  • ---- Coffee Break ----
  • Presentations from participants - schedule TBD
Registration Deadline:
July 15th, 2025
Register here now
Additional Information:

Workshop Materials | Contacts | Parking Info | Travel to Missoula | Registration | Directions